Journal: Drug Design, Development and Therapy

Article Title: Integrating Network Pharmacology and Component Analysis to Study the Potential Mechanisms of Qi-Fu-Yin Decoction in Treating Alzheimer’s Disease

doi: 10.2147/DDDT.S402624

Figure Lengend Snippet: Primer Sequence of Target Gene and Internal Reference Gene

Article Snippet: D-galactose was purchased from Shanghai Macklin Biochemical Co., Ltd. Piracetam was purchased from Tianjin Jinshi Pharmaceutical Co., Ltd; The primary antibodies for Bcl-2, Caspase-1, Bax, PSD95, SYP and β-actin were purchased from Wuhan Boster Biological Technology Co., Ltd. IL-1β, IL-18 and TNF-α ELISA kits were obtained from Elabscience Biotechnology Co., Ltd. DAPI purchased from Beijing Solarbio Science & Technology Co., Ltd.

Techniques: Sequencing

Journal: Drug Design, Development and Therapy

Article Title: Integrating Network Pharmacology and Component Analysis to Study the Potential Mechanisms of Qi-Fu-Yin Decoction in Treating Alzheimer’s Disease

doi: 10.2147/DDDT.S402624

Figure Lengend Snippet: The effect of QFY on synapses and neuronal apoptosis. ( A ) The expression of PSD95 and SYP protein in mice hippocampus. ( B ) Amplification curve, fusion curve and mRNA expression of PSD95 and SYP. ( C ) The expression of Bax, Bcl-2, and Caspase-1 protein in mice hippocampus. n = 5 in each group, ** P < 0.01 vs the con group; ## P < 0.01 vs the model group.

Article Snippet: D-galactose was purchased from Shanghai Macklin Biochemical Co., Ltd. Piracetam was purchased from Tianjin Jinshi Pharmaceutical Co., Ltd; The primary antibodies for Bcl-2, Caspase-1, Bax, PSD95, SYP and β-actin were purchased from Wuhan Boster Biological Technology Co., Ltd. IL-1β, IL-18 and TNF-α ELISA kits were obtained from Elabscience Biotechnology Co., Ltd. DAPI purchased from Beijing Solarbio Science & Technology Co., Ltd.

Techniques: Expressing, Amplification

Journal: Nature Communications

Article Title: Principal cell activity induces spine relocation of adult-born interneurons in the olfactory bulb

doi: 10.1038/ncomms12659

Figure Lengend Snippet: ( a ) Experimental procedure for chronic in vivo two-photon imaging. ( b ) Example of GC dendrite images acquired on 2 consecutive days. ( c ) Higher magnification of the boxed area in b . Time-lapse two-photon imaging of relocating spine during the first imaging day (upper panel). Note that the same spine is stabilized at the relocated position and that no displacement is observed during the second imaging day. The first and last z-stacks have been false-coloured in red and green to compare the first and last images of the time-lapse sequence, respectively. ( d ) Another example of relocating spine. ( e ) In vivo images showing the spine relocation of an adult-born GC (lower panels). At the end of the in vivo imaging, the animal was perfused, and OB sections were prepared for anti-synaptophysin immunohistochemistry. Confocal image showing the dendrite of the imaged GC (upper middle panel) and the anti-synaptophysin labelling (upper right panel). Note that spine relocating in vivo is directly opposed to synaptophysin puncta. Scale bars: 20 μm for b 2 μm for c , d ; 5 μm for e centre panel and 2 μm for e right panels.

Article Snippet: Anti-synaptophysin antibody was coupled to a fluorescent antibody using Mix-n-Stain CF568 antibody labelling kits (Biotium).

Techniques: In Vivo, Imaging, Sequencing, In Vivo Imaging, Immunohistochemistry